HLA-B27 Typing Using Simple Allele Specific PCR in Suspected | 54897

జర్నల్ ఆఫ్ ఆర్థరైటిస్

ISSN - 2167-7921


HLA-B27 Typing Using Simple Allele Specific PCR in Suspected Spondyloarthritis Patients Visiting Different Hospitals of Kathmandu

Anil Kumar Sah, Pragya Hatawal, Sandip Shrestha, Laxmi Sharma, Laxmi Sen Thakuri, Riju Dahal, Shreeya Parajuli, Prabin Nepal, Pravesh Rajbhandari and Basant Pant

Background: Human leucocyte antigens-B27 is products of genes found at
the human major histocompatibility complex class I molecule, associated
with a several rheumatic disease. There is a marked variation of association
between spondyloarthritis and HLA B27 surface antigen. This association
encouraged in testing of HLA-B27 antigen as one of the diagnostic tools
in this inflammatory disease. The study was conducted with the aim to
perform HLA-B27 typing by means of simple allele specific PCR in suspected
spondyloarthritis (SpA) patients.
Methods: The whole blood samples of the suspected SpA patients were
collected from different hospitals and DNA was extracted. HLA-B27 Typing
was performed by sequence specific primer PCR (SSP-PCR).
Results: A total 112 and 25 sample of suspected spondyloarthritis and healthy
population were involved in this study, Out of 112 suspected spondyloarthritis
patients 23.2% (n=26/112) were HLA-B27 positive; similarly in 25 healthy
specimens 4% (n=1/25) were positive for HLA-B27. HLA-B27 were positive
in 69.2% and 30.8% (8/26) in the 15-40 and >40 years age group respectively
(p=0.54), and 76.9% and 23.1% in male and female respectively (p=0.15).
Clinical characteristics; inflammatory low back pain, morning stiffness and
enthesitis of the patient were significantly associated with HLA-B27 Typing
Conclusion: Present research output confirmed the significance of HLA-B27
allele as a novel and rapid molecular marker for diagnosis of spondyloarthritis
also in Nepalese patients.