Alireza Mohammadyari, Seyedeh Tahereh Razavipour, Maryam Mohammadbeigi, Masoud Negahdary, Marziyeh Ajdary
"This study aims to suggest the limits of copper oxide nanoparticle uses for medicinal purposes and was performed to investigate the effect of different doses of copper oxide nanoparticle in rats. A total of 32 healthy male Wistar rats of the same age (12 weeks old) weighing 250-280 g were used. Animals were randomly divided into groups, two GNP-treated rat groups and one control group. The 100 of 200 and 400-ppm copper oxide nanoparticles was intra-peritoneally administered in rats for exposure duration of 15 days. Then, several biochemical parameters such as aspartate aminotransferase, alanine transaminase, alkaline phosphatase, creatinine, fast blood sugar, albumin, blood urea nitrogen, total protein were evaluated. Significant changes were observed (p < 0.05) in biochemical parameters in the 400-ppm groups. The changes were nonsignificant in the other groups (200,100-ppm group). In the 40 ppm group, a significant increase was also found in liver function enzymes like ALT and AST (p < 0.05), ALP (p < 0.05), creatinine, albumin, total protein, blood urea nitrogen, fast blood sugar (p < 0.05). This study indicates that the CuO nanoparticles in doses (<400ppm) is safe for biomedical application and has no side effects, but its high dose (≥ 400 ppm) is toxic."